Search results for "Indirect Transmission"

showing 2 items of 2 documents

First molecular detection of mycobacterium bovis in environmental samples from a French region with endemic bovine tuberculosis

2016

Aims The aim of the study was to determine the prevalence of Mycobacterium bovis (the causative agent of bovine tuberculosis, bTB) in environmental matrices within a French region (Cote d'Or) affected by this zoonotic disease. Methods and Results We report here the development and the use of molecular detection assays based on qPCR (double fluorescent dye labelled probe) to monitor the occurrence of Mycobacterium tuberculosis complex (MTBC) or M. bovis in environmental samples collected in pastures where infected cattle and wildlife had been reported. Three qPCR assays targeting members of the MTBC (IS1561’ and Rv3866 loci) or M. bovis (RD4 locus) were developed or refined from existing ass…

0301 basic medicineGenotype040301 veterinary sciences[SDV]Life Sciences [q-bio]030106 microbiologyIndoor bioaerosolAnimals WildLocus (genetics)Applied Microbiology and BiotechnologyMicrobiology0403 veterinary scienceFeces03 medical and health sciencesGenotypeEnvironmental MicrobiologyMustelidaePrevalenceBovine tuberculosisAnimals[SDV.BV]Life Sciences [q-bio]/Vegetal Biologyquantitative real-time PCRbovine tuberculosisFeces2. Zero hungerMycobacterium bovisbiologyfungi04 agricultural and veterinary sciencesGeneral MedicineContaminationbiology.organism_classificationMycobacterium bovis3. Good healthMycobacterium tuberculosis complex[SDE]Environmental SciencesCattleindirect transmissionFranceTuberculosis BovineenvironmentBiotechnology
researchProduct

Prolonged survival of Puumala hantavirus outside the host: evidence for indirect transmission via the environment

2006

The capability of rodent-borne viruses to survive outside the host is critical for the transmission dynamics within rodent populations and to humans. The transmission of Puumala virus (PUUV) in colonized bank voles (Clethrionomys glareolus) was investigated and additional longevity studies in cell culture with PUUV and Tula (TULV) hantaviruses were performed. Wild-type PUUV excreted by experimentally infected donor bank voles was shown to be transmitted indirectly between rodents through contaminated beddings, and maintained its infectivity to recipient voles at room temperature for 12–15 days. In cell culture supernatants, PUUV and TULV remained infectious for 5–11 days at room temperature…

MaleTime FactorsIndirect TransmissionPuumala virusViruslaw.inventionMice03 medical and health scienceslawVirologyChlorocebus aethiopsAnimalsVero Cells030304 developmental biologyHantavirusInfectivity0303 health sciencesbiologyArvicolinae030306 microbiologyHost (biology)Temperaturebiology.organism_classificationHousing AnimalVirology3. Good healthDisease Models AnimalTransmission (mechanics)Hemorrhagic Fever with Renal SyndromeEquipment ContaminationFemalePuumala virusBunyaviridaeJournal of General Virology
researchProduct